Evaluation of murinetriple negative breast cancer cell viability exposed to magnetic nanoparticles

MARÍA MARTA FACCHINETTI; ALEJANDRO CURINO; VERÓNICA LASSALLE; MARÍA JULIA FERRONATO; MARIELA AGOTEGARAY

Congreso; Congreso Nanomed-2020 First Zooming into Preclinical Nanomedicines in the Era of COVID-19; 2020

Asociación Argentina de Nanomedicinas

INTRODUCTION. Magnetic nanoparticles (MNPs) represent a tool forlocalized therapeutics on specific sites of the body by the influence of anexternal magnetic field. They may act as drug carriers as well as contrastagents for resonance magnetic images and agents for hyperthermiatreatment in different oncological pathologies. In this work, we developedMNPs coated with different agents in order to induce biocompatibleplatforms for future loading of chemotherapies. Their effects on cellviability of murine triple negative breast cancer cells (TNBC) were studiedto provide novel information for possible future clinical applications.MATERIALS AND METHODS: MNPs were synthesized by co-precipitationmethod from iron precursors and different coating agents: citric acid,glutamic acid, adenine, oleic acid, beta-cyclodextrin, and 3-aminopropyltriethoxysilane (APTES). Physicochemical characterizationwas performed employing FTIR spectroscopy, analysis of hydrodynamicdiameter and zeta potential, transmission electronic microscopy and ironcontent. Cell viability of murine 4T1TNBC cells exposed for 24 h to differentconcentrations of MNPs (0-500 μg/mL) was evaluated by crystal violetstaining assays. The experiments were repeated twice and performed intriplicate.RESULTS. Spectroscopic studies demonstrated the successful coating of the different MNPs with the coating agents employed. Physicochemical properties of MNPs were proper for biomedical applications in terms of hydrodynamic diameters (between 200 and 350 nm) and stability in physiological media pH near 7 (PDI