Bioctive Flavonoids Isolated from Atriplex undulata (Moq) D. Dietr. (Chenopodiaceae)

SUEIRO R.A; RODRIGUEZ S.A; LEIRO J.M.; MURRAY A.P

Panama

Simposio; II International Symposium on Chemistry, Biology and Pharmacological properties of Natural Products, IX Iberoamerican Symposium on Medicinal Plants and the IV Iberoamerican Symposium on Cancer Research; 2019

INDICASAT AIP

BIOACTIVE COMPOUNDS ISOLATED FROM Atriplex undulata (Moq) D. Dietr. (Chenopodiaceae), AS A SOURCE OF THERAPEUTIC AGENTS.Rodriguez S.A.1,2, Sueiro R.A.3, Murray A.P.1 and Leiro J.M.21 INQUISUR, Departamento de Química, UNS, Bahía Blanca, Argentina.2 IIAA, Laboratorio de Parasitología, USC, Santiago de Compostela, Spain3 IIAA, Labotarorio de Microbiología, USC, Santiago de Compostela, Spain. Email: sarodrig@uns.edu.arAtriplex undulata is an endemic plant occurring in Argentina Patagonia region. Popularly it is known as "Zampa Crespa" and is used as astringent and antiequimotic.This work describes the chemical composition and the biological properties of new glycosidated flavonoid (1) and their aglycone (2) isolated from aerial parts of A. undulata.The flavonoids (1-2) were obtained by different chromatographic procedures from the EtOAc subextract (EtOAc) that were identified and characterized by 1H and 13C NMR (1D and 2D experiments).The active compounds, isorhamnetin-3,7- O- diglucopyranoside-4´-O-galactoside (1) and isorhamnetin (2) were evaluated in different biological assays.The anti-inflammatory effect was evaluated by measuring release of interleukins, tumor necrosis factor, expression of inducible nitric oxide synthase and cyclooxygenase-2 by reverse transcriptase-polymerase chain reaction.The inhibition of mutagenicity was evaluated using the Ames assay with 5 strains of Salmonella Typhimurium in the absence and presence of exogenous metabolic activation (rat liver fraction S9), against different mutagens.The antimicrobial activity was assessed against gram-positive and gram-negative bacteria and fungi.The EtOAc, 1 and 2 were active against all microorganisms and showed antioxidant activity by scavenging free radicals. Also, the results demonstrate that 1 and 2 exhibit potent anti-inflammatory and antimutagenic activities. These compounds are able to reduce or inhibit DNA lesions or mutations that induce cancer cells and diseases caused by genotoxic agents. These effects were previously observed for the crude extract of this plant.The inhibition produced by 1 and 2 isolated from the EtOAc A. undulata, could be interesting for the prevention of several diseases and as their therapeutic efficacy.