Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials

GRAVINA ANABELA NOEL; WELMAN MÉLANIE; MAGHNI KARIM; MESSINA PAULA VERÓNICA; YAHIA L'HOCINE; MBEH DORIS ANTOINETTE

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 1860 p. 452 - 464

0304-4165

Background: Cerium oxide (CeO2) and Ce-doped nanostructured materials (NMs) are being seen as innovativetherapeutic tools due to their exceptional antioxidant effects; nevertheless their bio-applications are still intheir infancy.Methods: TiO2, Ce?TiO2 and CeO2?TiO2 NMs were synthesized by a bottom-up microemulsion-mediatedstrategy and calcined during 7 h at 650 °C under air flux. The samples were compared to elucidate thephysicochemical characteristics that determine cellular uptake, toxicity and the influence of redox balancebetween the Ce3+/Ce4+ on the cytoprotective role against an exogenous ROS source: H2O2. Fibroblasts wereselected as a cell model because of their participation in wound healing and fibrotic diseases.Results: Ce?TiO2 NM obtained via sol?gel reaction chemistry of metallic organic precursors exerts a realcytoprotective effect against H2O2 over fibroblast proliferation,while CeO2 pre-formed nanoparticles incorporatedto TiO2 crystalline matrix lead to a harmful CeO2?TiO2 material. TiO2 was processed by the same pathways asCe?TiO2 and CeO2?TiO2 NM but did not elicit any adverse or protective influence compared to controls.Conclusions: It was found that the Ce atoms source and its concentration have a clear effect on material'sphysicochemical properties and its subsequent influence in the cellular response. It can induce a range of biologicalreactions that vary from cytotoxic to cytoprotective.General significance: Even though there are still some unresolved issues and challenges, the unique physical andchemical properties of Ce-based NMs are fascinating and versatile resources for different biomedical applications.