INQUISUR 21779
INSTITUTO DE QUIMICA DEL SUR
Unidad Ejecutora - UE
artículos
Título:
Flow-batch analyser for the chemiluminescence determination of catecholamines in pharmaceutical preparations
Autor/es:
MARCOS GRUNHUT; VALDOMIRO L. MARTINS; MARIA E. CENTURIÓN; MÁRIO C.U. DE ARAÚJO; BEATRIZ S. FERNÁNDEZ BAND
Revista:
ANALYTICAL LETTERS
Editorial:
Taylor & Francis
Referencias:
Lugar: Londres; Año: 2011 vol. 44 p. 1 - 15
ISSN:
0003-2719
Resumen:
A novel, simple, cheap, flexible, versatile, and highly sensitivity flow-batch analyzer (FBA) with chemiluminescence detection was developed for determination of dopamine, norepinephrine, and epinephrine in pharmaceutical preparations. The method was based on the inhibitory effect of the mentioned catecholamines on a luminol-potassium hexacyanoferrate (III) chemiluminescence system in alkaline medium. The optimization of the chemical variables affecting this chemiluminescence inhibition effect has been carried out using a Box-Behnken experimental design. The sample throughput was 28 h-1. The system allowed the automatic preparation of standard solutions and analytical process can be accomplishedjust by changing the operational parameters in FBA control software.
