INVESTIGADORES
FURLAN Ricardo Luis Eugenio
artículos
Título:
Thin Layer Chromatography-Autography-High Resolution Mass Spectrometry Analysis: Accelerating the Identification of Acetylcholinesterase Inhibitors
Autor/es:
RAMALLO, IA; SALAZAR, MO; FURLAN, RLE
Revista:
PHYTOCHEMICAL ANALYSIS
Editorial:
JOHN WILEY & SONS LTD
Referencias:
Lugar: Londres; Año: 2015 vol. 26 p. 404 - 412
ISSN:
0958-0344
Resumen:
A new strategy for identification of AChE inhibitors present in mixtures employing High Resolution Mass Spectrometry and a Thin Layer Chromatography-biological staining was developed. The strategy was used to detect the presence of physostigmine in an extract of Brassica rapa L. spiked with the inhibitor. Similarly, caffeine was straightforwardly spotted as responsible for the inhibitory properties of an extract of Ilex paraguariensis Saint-Hilaire. This paper illustrates the feasibility of assigning molecular formulas of active compounds in mixtures directly from autography.
