A method to obtain monosporic cultures of arbuscular mycorrhizal fungi.

FRACCHIA, S., MENENDEZ, A., GODEAS, A.. & OCAMPO J.A..

SOIL BIOLOGY AND BIOCHEMISTRY

elsevier

Lugar: amsterdam; Año: 2001 vol. 33 p. 1283 - 1285

0038-0717

 We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. Sp., Glomus mosseae andG. Sp were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0,04g Gel Gro. The contents of a vermiculite-perlite (1/1, v/v) dish with 2-week-old clover seedlings were transferres onto the Gel-Gro medium with the germinated spore. All plants inoculated with single germinated spores developed mycorrhizas. With this system, n on destructive observation of development of hyphae, formation of fan-like structures and penetration of AM fungi into the plant roots was possible.