Interactions of rutin with oxidovanadium(IV) cation. Anticancer improvement effects of glycosylated flavonoids.

GOITIA, HELEN; QUISPE, PATRICIA; NASO, LUCIANA; MARTÍNEZ, VALERIA; REY, MARILIN; RIZZI, ALBERTO; FERRER, EVELINA; WILLIAMS, PATRICIA ANA MARÍA

NEW JOURNAL OF CHEMISTRY

ROYAL SOC CHEMISTRY

Año: 2019

1144-0546

This work reports the biological evaluation of the new complexNa2[VO(rut)(OH)2].5H2O (rut= rutin, glycosylated flavonoid). The complex resulted different in its coordination mode (catechol-like) than those previously reported, [VO(rutin)(H2O)2]2(SO4).4H2O and [VO(rut)2].4H2O (acetylacetonate-like coordination). Due to the coordination mode, the complex only improved the antioxidant activity of the ligand against superoxide and hydroxyl radicals. Results show that while both 100 μM rutin and V(IV)O did not exhibit cytotoxic activity on A549 cells, the complex selectively improved the anticancer effect (IC50 = 95 μM), cellular reactive oxygen species (ROS) generation and depletion of the nonenzymatic antioxidant glutathione (GSH) producing oxidized glutathione (GSSG) and it did not affect viability of normal embryonic lung cell line (MRC-5) (up to 100 μM). All these data, and given the reversion of the cell killing effect of the complex upon treatment with the antioxidant agent N-acetylL-cysteine (NAC), suggested a stress oxidative mechanism. Rutin and VOrut can spontaneously bind bovine serumalbumin (BSA) and they can be stored and transported by the protein.