Immobilization of catalase from Aspergillus niger on inorganic and biopolymeric supports for H2O2 decomposition

ANDREA EBERHARDT; VIVIANA PEDRONI; MARÍA ALICIA VOLPE; MARÍA LUJÁN FERREIRA

APPLIED CATALYSIS B-ENVIRONMENTAL

Año: 2004 vol. 47 p. 153 - 163

0926-3373

This paper studies the H2O2 decomposition using supported catalase. The supports are g -Al2O3, FeCl2 treated g -Al2O3, glutaraldehyde treated chitosan and glutaradehyde treated cellulose. Catalase/g -Al2O3 is inactive, but the treatment with FeCl2 renders the oxide active. The Extended Hückel type analysis (EHMO) indicates that the alumina-catalase interaction is strong, suggesting that a denaturation of the enzyme is accomplished when supporting on this oxide. Amongst all the samples, catalase/glutaraldehyde-cellulose is the most active, especially after 6 month of storage. After aging, catalase/glutaraldehyde-chitosan becomes inactive, probably due to the fact that NH2 groups on chitosan react with the enzyme active sites.