INVESTIGADORES
FERNANDEZ CIRELLI Alicia
artículos
Título:
Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer
Autor/es:
E. OLIVA; A. FERNÁNDEZ CIRELLI; R. MUCHNIK DE LEDERKREMER
Revista:
CARBOHYDRATE RESEARCH
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 1985 vol. 138 p. 257 - 266
ISSN:
0008-6215
Resumen:
A glycopeptide (In1) was isolated by phenol-water extraction from Cyttaria harioti Fischer, parasite of Nothofagus sps. Neutral sugars account for 89% of In1 and were characterized as glucose, mannose, and galactose. Glucosamine, identified by g.l.c., was colorimetrically estimated (5.8%). The molar ratio of Glc:Man:Gal:GlcNAc was 17:11:3:2. The linkages between the various monosaccharide residues were established through methylation analysis and periodate oxidation studies. The anomeric configurations of the various glycosyl groups were determined by chromium trioxide oxidation of the acetylated polysaccharide. The results were confirmed by 13C-n.m.r. spectroscopy. The sugar chain is N-glycosyl-linked to the peptide. Structural features of the carbohydrate moiety of glycopeptide In1 are described.
