INVESTIGADORES
FERNANDEZ Liliana Patricia
artículos
Título:
FLOW INJECTION SPECTROFLUORIMETRIC DETERMINATION OF CARVEDILOL MEDIATED BY MICELLES
Autor/es:
SILVA, RAUL; WANG, CHIEN CHUN; FERNANDEZ, LILIANA; MASI, ADRIANA
Revista:
TALANTA
Editorial:
Elsevier
Referencias:
Año: 2008 p. 166 - 171
ISSN:
0039-9140
Resumen:
P { margin-bottom: 0.21cm; }
A novel flow injection
(FI)-spectrofluorimetric methodology for the determination of
carvedilol in micro-
heterogeneous medium has been
developed. In the sodium dodecyl sulfate (SDS) surfactant medium,
an additional fluorescence enhancement
was produced by the electrolyte NaCl. A total enhancement of
3.1-fold in the native fluorescent
response was achieved respect to aqueous medium. Using an excitation
and emission wavelength of 286 and 341
nm, respectively, a good linear relationship was obtained in the
range of 9 × 10−8 to 1 × 10−6 mol
L−1 with a detection limit of 3.63 × 10−9 mol L−1 (S/N = 3).
This method was
applied to determine carvedilol in
commercial pharmaceutical formulations. Good concordance was found
between the nominal (6.25, 12.5 and
25.0 mg) and experimental values. The new methodology developed
showed high selectivity respect to the
common excipients used in pharmaceuticals. The sampling rate
was 30 samples h−1 . From the
fluorescent properties, binding constant for carvedilol?SDS
determined was
3.2 × 102 L mol−1 .