INVESTIGADORES
FERNANDEZ Liliana Patricia
artículos
Título:
O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry
Autor/es:
PERALTA, CECILIA; FERNANDEZ, LILIANA
Revista:
Journal Pharmaceutical Analysis
Editorial:
Elsevier
Referencias:
Año: 2014 vol. 4 p. 368 - 373
ISSN:
2095-1779
Resumen:
A simple,eco-friendly,sensitiveandeconomic flow injectionspectrofluorimetric methodwas developed forthedeterminationofO-(β-hydroxyethyl)rutosides. Theprocedurewasbasedontheuseof an anionicsurfactantsuchassodiumdodecylsulfatetoprovideanappreciableO-(β-hydroxyethyl) rutosides fluorescence enhancement,increasingconsiderablythesensitivityofdetection.Allthevariables affecting the fluorescence intensitywerestudiedandoptimized.The flow ratewas5mL/minwith detection at450nm(afterexcitationat346nm).Alinearcorrelationbetweendrugamountandpeakarea was establishedforO-(β-hydroxyethyl)rutosides intherangeof0.01?200 mg/mL withadetectionlimitof 0.001 mg/mL (s/n¼3). Validationprocesseswereperformedbyrecoveringstudieswithsatisfactory results. ThenewmethodologycanbeemployedfortheroutineanalysisofO-(β-hydroxyethyl)rutosides in bulks aswellasincommercialformulations.
