INVESTIGADORES
FARIÑA Julia Ines
artículos
Título:
Characterization of the oxidative enzyme potential of wild white rot fungi from Misiones subtropical rainforest (Argentina)
Autor/es:
M.I. FONSECA; P.D. ZAPATA; L.L. VILLALBA; JULIA I. FARIÑA
Revista:
Acta Biológica Colombiana
Editorial:
Universidad Nacional de Colombia
Referencias:
Lugar: Bogotá; Año: 2015 vol. 20 p. 47 - 47
ISSN:
1900-1649
Resumen:
ABSTRACTThis research aimed to evaluate the potential of several native white rot fungi (WRF) isolated from subtropical environments of Misiones (Argentina) to produce different ligninolytic enzymes. Coriolus versicolor f. antarcticus BAFC 266, Pycnoporus sanguineus BAFC 2126 and Phlebia brevispora BAFC 633 showed the highest phenoloxidase activity. Ganoderma applanatum strain E, P. sanguineus BAFC 2126 and P. brevispora BAFC 633 revealed marked laccase and peroxidase activity. C. versicolor f. antarcticus, G. applanatum (strain A) and Trametes villosa, gave high positive reactions with 2,6-dimethoxyphenol oxidation at the lowest tested pH. C. versicolor f. antarcticus, G. applanatum strains D and F, T. elegans BAFC 2127and T. villosa, showed the highest manganese peroxidase activity. C. versicolor f. antarcticus also produced the highest lignin peroxidase activity. Tyrosinase activity was mostly evident in G. applanatum strains (D and F) and Phanerochaete chrysosporium HHB 11741. Kraft liquor decolorization results were variable and depended on the fungus and the liquor concentration. Some fungi with moderate ligninolytic activity showed high decolorization rates (e.g. Pleurotus sajor-caju and Steccherinium sp. BAFC 1171) indicating the significance of additional approach to evaluate a potential biotechnological application.Keywords: oxidative enzymes, screening, white-rot fungi.
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