INVESTIGADORES
FARIÑA Julia Ines
artículos
Título:
Cost-effective optimized scleroglucan production by Sclerotium rolfsii ATCC 201126 at bioreactor scale. A quantity-quality assessment
Autor/es:
VALDEZ, ALEJANDRA L.; DELGADO, OSVALDO D.; FARIÑA, JULIA I.
Revista:
CARBOHYDRATE POLYMERS
Editorial:
ELSEVIER SCI LTD
Referencias:
Año: 2021 vol. 260
ISSN:
0144-8617
Resumen:
Exopolysaccharide (EPS) secretion by Sclerotium rolfsii ATCC 201126 in submerged cultures, already identified as high-osmolarity responsive, was assessed by reducing C-source without compromising EPS yields. A designed medium with 80 g sucrose L−1 (MOPT80) was tested at 3 L-bioreactor scale at different temperature, agitation, aeration and pH (uncontrolled vs. controlled) values. Optimal operative conditions (200 rpm, 28 °C, 0.5 vvm and initial pH -pHi- 4.5) were validated, as well as the possibility to work at pHi 5.5 to reduce biomass production. Purified EPSs produced in MOPT80 at optimal and other valid operative conditions exhibited refined grade (
