Osmolar stress altered the differentiation of extravillous trophoblast pathway

MATÍAS N. SIERRA; JULIETA REPPETTI; CAROLINA ANUD; MARIANA FARINA; ALICIA DAMIANO; MARTINEZ NORA

Ciudad Autónoma

Congreso; IFPA 2019- VIII LatinAmerican Symposium on Maternal-Fetal Interaction & Placenta; 2019

Objetive: to explore the effect of hyperosmolarity on the extravillous trophoblast cells migration, invasion and endovascular differentiation.Methods: Swan-71 cell line (human extravillous trophoblast cells) was cultured in complete DMEM-F12 and different concentrations of sucrose were added to generate the osmolar stress.Cell migration was evaluated by wound healing assay, activity of metalloproteinases (MMPs) by zymography, cell invasion was evaluated in transwells pre-coated with Matrigel® and endovascular differentiation was analyzed by the formation of tube-like structures in plates coated with Matrigel®.Results: In all the conditions tested cell viability was not modified. Hyperosmolarity significantly reduced cell migration, MMPs activity and cell invasion in Swan-71 cells (33 ± 4%, 75 ± 8%, and 45 ± 6%, respectively compared to iso-osmolar condition; p