INVESTIGADORES
FANOVICH Maria Alejandra
congresos y reuniones científicas
Título:
A NOVEL LITHIUM-DOPED 45S5 BIOACTIVE GLASS WITH POTENTIAL TO STIMULATE ANGIOGENESIS
Autor/es:
VARGAS, GE; HARO DURAND, LA; VERA MESONES, R; FANOVICH, MA; GORUSTOVICH, A
Lugar:
Madrid
Reunión:
Congreso; Conference on Cardiovascular Development, Disease and Repair; 2013
Institución organizadora:
CNIC
Resumen:
Recent experimental evidence
shows that lithium (Li) stimulates the in
vitro secretion of growth factors with pro-angiogenic activity. The
controlled and localized release of Li ions from bioactive glasses might prove
a promising therapeutic alternative to initiate angiogenesis and achieve
neovascularization in tissue repair and/or regeneration procedures. The aim of
this work was to assess the angiogenic effects of the ionic dissolution
products of new bioactive glasses based on 45S5-type glass (% weight): 45% SiO2, 24.5% Na2O,
24.5% CaO, 6% P2O5 doped
with 1% (45S5.1Li) or 5%
Li2O (45S5.5Li). The ionic
dissolution products were obtained by incubating 1% w/v particles (<5
µm) of bioactive glass in egg water at 37ºC in an orbital shaker for 72 h. The
determination of soluble ions lixiviated from the bioactive glasses was
conducted through inductively coupled plasma mass spectrometry (ICP-MS). In vivo angiogenesis assays
were carried out on dechorionated zebrafish embryos (Danio rerio) at 48 h post fecundation
(hpf). The embryos were incubated for 24 h at 28.5°C in 6-well culture
plates containing either 5 mL egg water (control) or egg water enriched with
the ionic dissolution products. We carried out 3 replicates with n=15 embryos
per treatment. The embryos were anesthetized with tricaine and processed for
the subsequent morphometric analysis of the subintestinal vascular plexus,
through enzyme-histochemical identification of endogenous alkaline phosphatase,
following the methodology described by Kamei et al (Methods Cell Biol 2010;
100:27-54). In all cases we observed the embryonic development expected for the
incubation time assessed. The results obtained showed that only the ionic
dissolution products of 45S5 bioactive glass doped with 5% Li2O
(45S5.5Li) exhibited pro-angiogenic capability. The positive effects can be
attributed to the release of 0.20
mM Li from 45S5.5Li microparticles.