Surface mixing of product and substrates of PLA2 in enzyme free mixed monolayers can reproduce enzyme-driven structure topography

LUISINA DE TULLIO; MARIA L. FANANI; BRUNO MAGGIO

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2013 vol. 1828 p. 2056 - 2063

0005-2736

It is well accepted that topographic changes in lipid monolayers hydrolized by lipolytic enzymes are a consequence of enzyme surface localization. Lateral packing deffects arrising from lipid phase coexistence are postulated places in which the enzyme is preferably localized. This work describes a method of mixing two lipid monolayers which simulate the manner that lipids are mixed when a lypolitic enzyme is hydrolizing the surface. In enzyme-free surface mixed film, a topographyc pattern similar to the generated actively by the enzyme was observed. The main conclusion from our experiments is that mixing-demixing properties of substrates and products of the enzymatic reaction determine topographyc evolution; enzyme surface localization is not the only factor.