EVALUATION OF CIRCULATING MONOCYTES AND PROINFLAMMATORY CYTOKINES IN PATIENTS WITH MOOD DISORDERS

ARENA, ÁNGELES R.; GRENDAS, LEANDRO N.; PENNA, MELINA B.; HUNTER, FERNANDO; OLAVIAGA, ALEJANDRO; PROKOPEZ, CINTIA; TIFNER, VERA; ARMESTO ARNALDO R; CARRERA SILVA, E. ANTONIO; DARAY, FEDERICO M.; ERRASTI, ANDREA E.

Buenos Aires

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

SAIC SAI AAFE Nanomed

Mood Disorder (MD) affects more than 300 million people globally, and its etiology is unknown. In recently published data, MD has been correlated with the inflammation and the immune system. This study aims to determine if there is a specific activation profile of monocyte-macrophage in patients with an MD that differentiates them from healthy control (HC), and to analyze if this activation profile in patients with MD is present only during a clinical episode of active depression (AD) or during no clinically active depression (NAD).Patients and HC were recruited and evaluated by psychiatrists using the International Psychiatry Interview MINI to diagnose the MD and the Hamilton Depression Rating Scale (HADRS) to define the 3 groups: AD, NAD, or HC. Blood samples were obtained and directly stained in 100 uL with the following cocktail of antibodies CD11b, HLA-DR, CD86, CD14, and CD16. IRB approved the study; each participant gave written consent. MD sample was 23% male, 77% female with a 25-62-year age range. Circulating monocytes and the proportion of the three subtypes were analyzed by flow cytometry-based on CD16negCD14++ (classical), CD16+CD14+ (intermediate), and CD16++CD14neg (non-classical). Additionally, the level of 16 cytokines was measured in plasma employing two panels of Legend Plex system by flow cytometry. Each panel was evaluated in a single sample of 50 uL of plasma. Our preliminary analysis shows that patients with MD (n=26) have a significantly reduced proportion of classical monocytes (p=0.02) and increased intermediate (p=0.02), and an upward trend in non-classical subsets (p=0.07) compared to the HC group (n=7). Moreover, we observed that patients with MD have higher concentrations of IL-1β (p=0.002), IFNγ (p=0.019), and IL-17 (p=0.004) than HC samples. After segregation of patients in AD (N=17) vs. NAD (N=12), no significant differences were observed yet, showing that some patients with MD have an inflammatory compromise even in remission.