DIFFERENTIAL EXPRESSION OF THE TAM COMPONENTS AND ITS CORRELATION WITH IFN PATHWAY IN HUMAN SMALL INTESTINE OF CELIAC PATIENTS

MARÍA LUZ IRIBARREN; FEDERICO PEREZ; CAROLINA N. RUERA; LUCIANA GUZMAN; LORENA MENENDEZ; LAURA GARBI; CINTHIA M. OLEXEN; JUAN MANUEL ORTIZ WILCZYÑSKI; ANDREA E. ERRASTI; E. ANTONIO CARRERA SILVA; FERNANDO G. CHIRDO

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

SAIC SAI SAFIS

Objective: Celiac Disease (CD) is a chronic inflammatory disease caused by an abnormal T cell-mediated immune response to the gluten in the diet in genetic susceptible individuals. The TYRO3, AXL and MERTK (TAM) receptors and their ligands (GAS6 and PROS1) play significant roles in the immune response but also in inflamma- tion and autoimmunity. Previously, we showed decreased PROS1 transcript levels along with increased GAS6 and AXL expression in duodenum of CD patients by RT-qPCR and immunofluorescence. Here, our aim was to determine the main TAM-expressing cell com- partment in CD and its relationship with the IFNs pathways, which are involved in both CD pathogenesis and the regulation of TAM expression. Material-Methods: Duodenal biopsies were collected from untreated CD patients (CD) and non-celiac (NC) individuals and analyzed by western blot (WB) and fluorescent microscopy us- ing specific immune linage markers. Principal component analysis (PCA) was also performed to correlate TAM axis component with IFNs pathways. Results: WB analysis showed that the expression of Pros1 and Gas6 has an inverse relationship in duodenum from CD patients. Furthermore, Gas6 was found increased in intraepithelial T cells (p-value