Extracellular mitochondria as a potential biomarker of pathological cardiac hypertrophy development

CAVALLI F; GODOY COTO J; VELEZ RUEDA , JO; VALVERDE CA; ENNIS IL

Congreso; Reunión anual de la Sociedad Argentina de Fisiología (SAFIS)- Asociación Latinoamericana de Ciencias Fisiológicas (ALACF) 2021; 2021

Introduction: several tissues are capable of releasing mitochondria into the bloodstream as a communication mechanism, increasing the release of dysfunctional mitochondria in some diseases. In pathological cardiac hypertrophy (PCH) development, the main organ affected is the heart, so it would be expected that most dysfunctional mitochondria in blood derive from it.Objective: to study extracellular mitochondria as a biomarker for the development of PCH induced by arterial hypertension. Methods: 9 and 12-month-old male spontaneously hypertensive rats were used. Cardiac function was evaluated by echo- and electrocardiogram. Mitochondria were isolated from the left ventricle and plasma to study the mitochondrial membrane potential with rhodamine-123. Western blots were conducted as isolation purity control. Transmission electron microscopy was performed in cardiac tissue. Results are expressed as mean±SEM (n) and compared by T-test, considering p