Evaluation of cell behavior on modified polypropylene with swift heavy ion irradiation

ARBEITMAN CR; IBAÑEZ IL; GARCÍA BERMÚDEZ G; DURÁN H; DEL GROSSO MF; SALGUERO NG; MAZZEI R

NUCLEAR INSTRUMENTS AND METHODS IN PHYSICS RESEARCH B - BEAM INTERACTIONS WITH MATERIALS AND ATOMS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2012 vol. 273 p. 51 - 54

0168-583X

Ion beam irradiation is a well-known mean to change the physic-chemical properties of polymers, and induced bio and cytocompatibility in controlled conditions and in selected areas. However, the enhancement of cell adhesion on a modified substrate does not mean that the surface is adequate for functional cells. The purpose of the present work is to study proliferation, changes in cytoskeleton and cell morphology on substrates as a function of irradiation parameters. We irradiated polypropylene with sulfur (S) ion-beam at energies of 110 MeV with fluences between 1 x 106 and 2 x 1010 ions cm-2. NIH3T3 cells were cultured on each sample. Cell morphology was observed using phase contrast microscopy and cytoskeleton proteins with fluorescence microscopy. The analysis show different cellular responses as a function of irradiation parameter and strongly suggests that different underlying substratum can result in distinct types of cytoskeleton reorganization.