Modulation of the hydration of lipid membrane phosphates by choline, glycerol and ethanolamine

LAIRION, FABIANA; DIAZ, SONIA; BRANDAN, SILVIA; BEN ALTABEF, AIDA; DISALVO, ANIBAL

Water properties of food pharmaceutical and biological materials

CRC Press

Lugar: Boca Raton ; Año: 2006; p. 503 - 512

<> We demonstrate using  Fourier Transform Infrared (FTIR) spectrometry and dipole surface potential in monolayers, that the hydration of the phosphate groups in lipid membranes depends on the chemical group esterified to the phosphate. The changes in the activity of an aqueous soluble enzyme in the presence of membranes of such as dimyristoylphosphatidylcholine (DMPC), dimyristoylphosphatidylethanolamine (DMPE), and dioleoylphosphatidyl choline plus phloretin (DOPC:Phlo) (1:1) was measured in correlation to the exposure of the phosphate of those compounds to the water phase. In consequence, the interaction of an aqueous soluble enzyme, such as an aspartyl protease from Mucor miehie, and its activity may be modulated by the groups anchored to the phosphate bias their hydration level as in lipid .