IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
artículos
Título:
R-Loop Mediated trans Action of the APOLO Long Noncoding RNA
Autor/es:
CHRIST, AURELIE; VELUCHAMY, ALAGURAJ; BLEIN, THOMAS; CRESPI, MARTIN; LUCERO, LEANDRO; JEGU, TEDDY; LATRASSE, DAVID; BENHAMED, MOUSSA; ARIEL, FEDERICO; MAMMARELLA, MARIA FLORENCIA; MARIAPPAN, KIRUTHIGA; LIU, CHANG
Revista:
MOLECULAR CELL
Editorial:
CELL PRESS
Referencias:
Año: 2020 vol. 77 p. 1055 - 1065
ISSN:
1097-2765
Resumen:
In eukaryotes, three-dimensional genome organization is critical for transcriptional regulation of gene expression. Long noncoding RNAs (lncRNAs) can modulate chromatin conformation of spatially related genomic locations within the nucleus. Here, we show that the lncRNA APOLO (AUXIN-REGULATED PROMOTER LOOP) recognizes multiple distant independent loci in the Arabidopsis thaliana genome. We found that APOLO targets are not spatially associated in the nucleus and that APOLO recognizes its targets by short sequence complementarity and the formation of DNA-RNA duplexes (R-loops). The invasion of APOLO to the target DNA decoys the plant Polycomb Repressive Complex 1 component LHP1, modulating local chromatin 3D conformation. APOLO lncRNA coordinates the expression of distal unrelated auxin-responsive genes during lateral root development in Arabidopsis. Hence, R-loop formation and chromatin protein decoy mediate trans action of lncRNAs on distant loci. Video Abstract: [Figure presented] In this work, Ariel et al. demonstrated that the APOLO long noncoding RNA can coordinate expression of multiple genes through sequence complementarity and R-loop formation in plants. Upon target recognition, APOLO can decoy protein complexes and modulate the three-dimensional chromatin conformation to fine-tune gene transcription.