Production of occlusion bodies of the Anticarsia gemmatalis multiple nucleopolyhedrovirus in serum-free suspension cultures of the saUFL-AG-286 cell line: influence of infection parameters and statistical optimization

GABRIELA A. MICHELOUD, VERÓNICA V. GIORIA, GUSTAVO PÉREZ, JUAN D. CLAUS

JOURNAL OF VIROLOGICAL METHODS

Elsevier

Año: 2009 vol. 162 p. 258 - 266

0166-0934

The influence of the parameters of infection, initial cell density (ICD) and multiplicity of infection (MOI) on the yield of occlusion bodies (OBs) of the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), produced in serum-free batch suspension cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 multiple nucleopolyhedrovirus (AgMNPV), produced in serum-free batch suspension cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 multiple nucleopolyhedrovirus (AgMNPV), produced in serum-free batch suspension cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), produced in serum-free batch suspension cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 (AgMNPV), produced in serum-free batch suspension cultures of the saUFL-AG-286 cell line, was investigated through two full factorial experiments with centre point. Both the maximum density of viable cells (MVCD) and the yield of occlusion bodies (OBY) reached in AgMNPV infected cultures proved to be sensitive to the modification of infection parameters. Maximum OBY as high as 3x108 OBs.mL-1 was attained provided that the MVCD was in the range between 4 and 8 x 105-1 was attained provided that the MVCD was in the range between 4 and 8 x 105 viable cells.mL-1. The optimum MVCD could be reached by a combination of several values of ICD and MOI. A regression model was established and validated in order to optimize the infection parameters. These results demonstrate the importance of an adequate selection of infection parameters in order to obtain high yields of baculovirus OBs in insect cell cultures, and they could be useful in the development of an in vitro feasible process to produce the AgMNPV insecticide in a new serum-free medium. produce the AgMNPV insecticide in a new serum-free medium. produce the AgMNPV insecticide in a new serum-free medium. values of ICD and MOI. A regression model was established and validated in order to optimize the infection parameters. These results demonstrate the importance of an adequate selection of infection parameters in order to obtain high yields of baculovirus OBs in insect cell cultures, and they could be useful in the development of an in vitro feasible process to produce the AgMNPV insecticide in a new serum-free medium. produce the AgMNPV insecticide in a new serum-free medium. produce the AgMNPV insecticide in a new serum-free medium. values of ICD and MOI. A regression model was established and validated in order to optimize the infection parameters. These results demonstrate the importance of an adequate selection of infection parameters in order to obtain high yields of baculovirus OBs in insect cell cultures, and they could be useful in the development of an in vitro feasible process to produce the AgMNPV insecticide in a new serum-free medium. produce the AgMNPV insecticide in a new serum-free medium. produce the AgMNPV insecticide in a new serum-free medium. The optimum MVCD could be reached by a combination of several values of ICD and MOI. A regression model was established and validated in order to optimize the infection parameters. These results demonstrate the importance of an adequate selection of infection parameters in order to obtain high yields of baculovirus OBs in insect cell cultures, and they could be useful in the development of an in vitro feasible process to produce the AgMNPV insecticide in a new serum-free medium. produce the AgMNPV insecticide in a new serum-free medium. produce the AgMNPV insecticide in a new serum-free medium. in vitro feasible process to produce the AgMNPV insecticide in a new serum-free medium.