INVESTIGADORES
DELGADO Osvaldo Daniel
congresos y reuniones científicas
Título:
Production and purification of MccP136, a cold-adapted antimicrobial produced by Serratia proteamaculans 136.
Autor/es:
SANCHEZ LA; SIÑERIZ F; DELGADO OD
Lugar:
Toronto
Reunión:
Congreso; SIM 2009; 2009
Institución organizadora:
Society for Industrial Microbiology
Resumen:
Antimicrobial
compounds are widespread in life forms to mediate competition, and their
industrial production could be important for the potential use as preservatives
in food, cosmetics and in pharmaceutical industries. Cold environments could be
a suitable source of microorganisms with ability to produce cold-active
antimicrobials of biotechnological interest.
A
psychotropic Serratia proteamaculans 136 strain producing MccP136 was
isolated from soil samples from Isla de los Estados (Tierra del Fuego,
Argentina). MccP136
production was optimized and a maximum of 800 AU ml-1 were obtained at early-stationary
phase of growth in batch fermentations carried out in a
BIOFLO 101 bioreactor with a working volume of 8.1 l with pH, temperature and
dissolved oxygen control. The compound was active in a broad range of pH
values (1 to 13) and presented an inhibitory effect against both Gram (+) and
Gram (-) microorganisms. A purification procedure was designed including
activated charcoal adsorption, solid phase extraction (SPE) and RP-HPLC under
semi-preparative conditions. The purity of the compound was checked by
analytical RP-HPLC and monitored by photodiode array detection and purification
parameters were calculated in each purification step. The molecular mass of the
antimicrobial compound (479.2 g/mol) was determined in a Hybrid Quadrupole-TOF
LC/MS/MS Mass Spectrometer by ion analysis. In conclusion, MccP136, a new cold-active
antimicrobial is active against diverse pathogenic strains, known as
etiological agents of common infections and food-borne diseases and it could be
important for applications in products that involve ?cold-steps? during
manufacture or processing.