Occurrence of a closely-related isolate to Maize yellow striate virus in wheat plants.

DUMÓN, A. D.; MATTIO, M. F.; ARGUELLO CARO, E. B.; ALEMANDRI, M. V.; PUYANÉ, N.; DEL VAS, M.; LÓPEZ LAMBERTINI, P; TRUOL, G.

AGRISCIENTIA

Universidad Nacional de Córdoba

Lugar: Córdoba; Año: 2015 vol. 32 p. 107 - 112

1668-298x

In Argentina, wheat fields have exhibited virus-like symptoms, such as chlorotic streaking, dwarfing, yellowing and empty ears since 2007. Symptomatic plants and leaves samples were collected in Marcos Juárez (2007) and Río Cuarto (2008 and 2013), both of them in Córdoba province. The virus was experimentally transmitted  from  symptomatic  wheat  plants  to  wheat  cv.  Baguette  10  and  cv. BioINTA 3005 using the vector Delphacodes kuscheli Fennah (Delphacidae); symptoms  of  chlorotic  streaking,  dwarfing  and  yellowing  appeared  in  the inoculated cereals at 10?15 days post-inoculation. Virus presence was confirmed by electron microscopy and RT-PCR using degenerated primers, which amplified a  conserved  region  of  the  plant  rhabdovirus  polymerase  (L)  gene.  Sequence comparison showed 98% nucleotide identity with Maize yellow striate virus C. Caroya (JQ715419) isolated from corn in Argentina. To our knowledge, this is the first report of the occurrence of Maize yellow striate virus in wheat in Argentina.