INVESTIGADORES
DEL VAS Mariana
artículos
Título:
Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat.
Autor/es:
BOSSIO, E.; DIAZ PALEO A; DEL VAS, M.; BAROLI, I.; ACEVEDO A; RÍOS, R. D.
Revista:
PLANT CELL TISSUE AND ORGAN CULTURE
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2013 vol. 112 p. 239 - 248
ISSN:
0167-6857
Resumen:
Somatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well
documented phenomenon and it has been shown through transcriptome analysis that
genes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis,
participate in the process. Thus, we investigated the influence of post-transcriptional
silencing (PTGS) of the glutathione biosynthesis genes GSH1 and GSH2 on somatic
embryogenesis in wheat. We found that PTGS of either of the target genes drastically
inhibits callus regeneration and overall efficiency of transformation, in a similar manner
as the GSH biosynthetic inhibitor buthionine sulfoximine. Supplementing the medium
with glutathione did not overcome the observed low efficiency of wheat transformation.
Furthermore, of the small number of obtained transformants, none exhibited altered
GSH1 and GSH2 levels of transcription. Thus, it is concluded that GSH is essential for
somatic embryogenesis and, as a consequence, it is difficult to regenerate wheat
plants with silenced GSH1 and GSH2 genes.