EFFECT OF NITRATIVE STRESS ON PLACENTAL AQP9 AND ITS ROLE IN THE PATHOGENESIS OF PREECLAMPSIA

MEDINA, YOLLYSETH; DI PAOLA, MAURICIO; ANUD, CAROLINA; REPPETTI, JULIETA; CASTRO-PARODI MAURICIO; DAMIANO, ALICIA E

Congreso; Reunión Anual de la Sociedad Argentina de Investigaciones Clínicas.; 2018

Preeclampsia is a gestational hypertensive syndrome of unknown etiology unique to human pregnancy. Increased levels of reactive nitrogen species (RNS) were described in preeclamptic placentas.RNS promotes the formation of peroxynitrite (ONOO-), that covalently modify proteins by nitration of tyrosine residues [3-nitrotyrosine (3NTp)] and lead to a non-functional protein. Nitration of a widerange of placental proteins were found increased in preeclampsia. Previously, in these placentas we reported an overexpression of AQP9 with a lack of functionality.Our aim was to evaluate the effect of nitrative stress mediated by ONOO- on placental AQP9 expression and functionality.This study was approved by the ethics committee of the Hospital Nacional Dr. Prof. A. Posadas. Explants from normal term placentas were cultured under conditions of nitrative stress induced by 100 μM de ONOO-. The cell viability, cytotoxicity, and cell damage indexes were evaluated. AQP9 expression and water uptake were also studied.The amount of 3NTp-AQP9 in trophoblast from preeclamptic and normal patients was determined using an immunoprecipitationassay. In the presence of 100 μM of ONOO-, AQP9 protein expression significantly increased (n=7; p