OSMOTIC STRESS AND HUMAN AMNION AQUAPORINS

DI PAOLA MAURICIO; DOROSSHNKO, BRIAN; SIERRA, MATÍAS; MASKIN BERNARDO; SZPILBARG, NATALIA; DAMIANO, ALICIA E

Congreso; Reunión Anual de la Sociedad Argentina de Investigaciones Clínicas.; 2018

Amniotic fluid (AF) is essential for normal fetal growth and development and aquaporins (AQPs) may be crucial in its regulation.To date, four AQPs (AQP1, AQP3, AQP8, AQP9) were described in human amnion and emerging evidence showed that an altered expression of these proteins might be associated with abnormal AF volume such as oligohydramnios or polyhydramnios. However, the etiologies of these syndromes are unknown. Recently, we demonstrated that AQPs facilitate water transportacross the human amnion, being the AQP1 the most important contributor. However, the effect of osmotic stress on the regulation of these proteins was not studied yet.Our aim was to study the effect of hypo and hyperosmolar stress on the expression and function of the human amnion AQPs.This study was approved by the ethics committee of the Hospital Nacional Dr. Prof. A. Posadas. Human amnion explants were cultured in hypo (150 mOsm) and hyperosmolar (400 mOsm) conditions generated by dilution of the culture medium or addition of a sucrose solution, respectively. Net transepithelial water movements across the human amnion were measured using a modified Ussing chamber.AQP1, AQP3, AQP8 and AQP9 expressions were assessed by Western Blot and semiquantitative RT-PCR. Hyperosmolar condition compared to isoosmolar condition, showed a significantly decreased in AQP1 and AQP9 expressions (n=7; p