HYPOXIA INDUCIBLE FACTOR-1 (HIF-1) IMPAIRS FUSION OF HUMAN TROPHOBLAST BEWO CELLS: INFLUENCE OF ENDOCANNABINOID SIGNALING.

ETCHEVERRY, TOMÁS; MARTINEZ, NORA; DAMIANO, ALICIA E; ABAN CYNTIA; FARINA MARIANA

Buenos AIres

Congreso; Reunion Conjunta de Sociedades de Biociencia; 2017

The syncytiotrophoblast (STB) form a multinucleated layer that ismaintained and regenerated by the fusion of underlying cytotrophoblastcells (CTBs). This structure is responsible for specialized functionsof the human placenta and disturbance in this process mayresult in pregnancy-associated diseases such as preeclampsia andintrauterine growth restriction. Hypoxia modifies human placentalvillous syncytialization, however these mechanisms remains unknown.Previously we demonstrated that hypoxia inducible factor-1alpha (HIF-1α) deregulated the endocannabinoid system (ES) in humanplacenta. In the present study, we investigated if HIF-1 and ESperform pivotal roles in trophoblast syncytialization.BeWo cell line was cultured with 100μM forskolin (FSK) to inducecell fusion. CTB (BeWo) and STB (BeWo + FSK) cells were treatedwith and without cobalt chloride (CoCl2), a hypoxia-mimetic agentthat stabilizes (HIF-1α); or R-(+)-Methanandamide (Met-AEA), astable anandamide analogous. Cell viability was evaluated by MTTassay. Markers for trophoblast syncytialization: syncytin-1 and glialcells missing-1 (GCM-1), were analyzed by western blot and RTPCR.The location of the E-cadherin was studied by immunocytochemistry.Incubation with CoCl2 and Met-AEA significantly reduced cell viability(p