ROLE OF THE CAVEOLAE IN THE REMODELING OF MATERNAL SPIRAL ARTERIES DURING THE FORMATION OF THE PLACENTA

REPPETTI, JULIETA; SEYAHIAN, ABRIL; RECA ALEJANDRA; BELTRAMONE, NATALIA; PARBORELL, FERNANDA; DAMIANO, ALICIA E; MARTINEZ, NORA

Congreso; Reunion Conjunta de Sociedades de Biociencia; 2017

Extravillous trophoblast (EVTs) cells of the human placenta migrate and invade the uterine decidua and adopt an endothelial phenotype to modify utero-placental arteries. These events also require a successful cooperation between EVTs and endothelial cells (ECs).Caveolae are membrane domains that compartmentalize intracellular signaling pathways to orchestrate different cellular events, as cell migration and invasion. However, the importance of caveolae during placentation remains unknown. Our aim was to evaluate the role of caveolae in EVTs migration, endovascular differentiation and interaction with ECs.Swan 71 cell line was treated with 5 mM methyl-β-cyclodextrin (MbCD) to disrupt caveolae. Conditioned medium (CM) was collected. Cav-1 expression was tested by Western blot and immunofluorescence. Migration was assessed by wound healing assay. Endovascular differentiation was evaluated by formation of tube-like structures in plates coated with Matrigel.To evaluate the interaction between EVTs and ECs (EA.hy926), proliferation (evaluated by MTT assay), migration and tube formation assays were performed in the presence of CM obtained from Swan 71 cells.Disruption of caveolae significantly decreased Cav-1 expression (n=5; p