INVESTIGADORES
DAMIANO Alicia Ermelinda
congresos y reuniones científicas
Título:
SHIGA TOXIN TYPE 2 AFFECTS HUMAN TROPHOBLAST FIRST TRIMESTER CELLS
Autor/es:
SCALISE, MARÍA LUJÁN; RECA, ALEJANDRA; DAMIANO, ALICIA E; SACERDOTI, FLAVIA; IBARRA CRISTINA
Lugar:
Puerto Varas
Reunión:
Simposio; VII LatinAmerican Symposium on Maternal-Fetal Interaction & Placenta; 2017
Institución organizadora:
SLIMP
Resumen:
Shiga toxin type 2 (Stx2) is the main virulence factor of Shiga toxin producingEscherichia coli (STEC). STEC are pathogens involved in food-borne diseases and are responsible for Hemolytic Uremic Syndrome development.We have previously demonstrated that Stx2 induce miscarriage and premature delivery in rats.Objective: The aim of this study was to evaluate the effects of Stx2 on human first trimester trophoblast cells.Methods: Swan71 and HTR-8 cells were exposed to different concentrations of pure Stx2 (1- 0.001 mg/ml) with or without lipopolysaccharide (LPS) 5 mg/ml in medium without serum (arrested conditions). Cell viability was evaluated at 24 h of Stx2-treatment by neutral red uptake.Migration was studied at 8 and 24 h by the wound-healing assay. Moreover, protein expression of matrix metalloproteinase 2 (MMP-2) was determined in the Swan71 supernatants at 24, 48, and 72 h in a gel zymography.Results: Stx2 did not affect cell viability in the HTR-8 and Swan71 lines even in the presence of LPS although impaired Swan71 cell migration after 24 h of treatment. Preliminary results showed that 1 mg/ml of Stx2 decreased the MMP-2 expression in Swan71 cells (39.9± 11.6%).Conclusion: These data indicate that Stx2 could affect trophoblast invasion and cause alterations in the maternal-placental interface and complications during gestation.