Study of membrane phospholipid composition on BeWo cells.

LEVI LORENA; ULLOA JERÓNIMO; NOVAK MARIANO; DAMIANO ALICIA E; STERIN-SPEZIALE NORMA

Rosario- Santa Fe;- Argentina

Congreso; XLII Reunion Anual de la Sociedad Argentina de investigacion Bioquimica y Biologia Molecular; 2006

Sociedad Argentina de investigacion Bioquimica y Biologia Molecular

Since the placenta is the first fetal arising organ that regulates fetal fatty acid homeostasis, the fatty acid/lipids composition in the placenta may serve as an indicator of fetal composition. It is known that Hypoxia inducible factor-1á (HIF-1á) is augmented in preeclapmsia. We observed an increase of AQP9 expression with a lack of functionality in this syndrome. The same results were observed when normal term explants were treated with Cl2Co, a HIF-1 inducer. The AQP-9 is a membrane-associated protein. It is well known that membrane protein activity can be modulated by the lipidic environment. Thus, alterations in lipid composition could induce changes in protein activity. Here we evaluated the phosphoslipidic composition of BeWo cell line membranes (accepted as a trophoblast human model). BeWo cells were exposed to a chemical hypoxia with Cl2Co. Lipid were extracted by Bligh-Dyer method, phospholipids separated by thin layer chromatography (TLC) and phosphatidylcholine (PC) and phosphatidylethanolamine (PE) quantified by Fiske-Subarrow. The biochemical viability was determined by â-hCG dosage. The PC/PE for control contidition was 3.76 ± 0.32 since the Cl2Co was 4.12 ± 0.028 (NS). Despite these results we can not be sure that changes in the phospholipidic composition causes the lack of functionality of AQP9.Further studies are needed to define the role of AQP9 in preeclampsia.