La hipoxia modularia la expresión de caveolina-1 en placenta humana.

RECA ALEJANDRA; SZPILBARG NATALIA; DIETRICH VALERIA; ZOTTA ELSA; DAMIANO ALICIA E

Buenos Aires

Jornada; XII Jornadas anuales de la Sociedad Argentina de Biología.; 2010

SAB

Increasing evidence indicates that O2 is a key regulator of trophoblast differentiation, and failure of the oxygen-associated developmental events contributes to placental disease such as preeclampsia. It has been suggested that alterations of cellular caveolin-1 levels may lead to different angiogenic responses. Caveolin-1 protein is expressed at very high levels in placental artery and villous microvessels. Previously, we have reported a reduced expression of caveolin- 1 in preeclamptic placentas. The aim of our work was to evaluate if different oxygen tensions may alter caveolin-1 expression in human placentas. Explants from normal term placenta were incubated in normoxia, hypoxia, and in hypoxia/reoxygenation (H/R). Semiquantitative RT-PCR, Western blot analysis and immunofluorescence were performed to study Caveolin-1 expression. We found that the expression of Caveolin-1 decreased significantly when explants were cultivated under H/R and it was almost undetectable in endothelial vessels. Our results may indicate that Caveolin-1 expression may be modulated by O2 levels and this could be associated to deficient trophoblast invasion of the endometrial arteries observed in preeclampsia.