IMPACT OF HYPEROSMOLARITY ON PLACENTAL ANGIOGENESIS AND CAVEOLIN-I EXPRESSION

REPPETTI, JULIETA; GORNALUSSE, GERMÁN; BELTRAMONE, NATALIA; DAMIANO ALICIA E; MARTINEZ, NORA

Buenos Aires

Congreso; Reunion Anual de la Sociedad Argentina de Fisiología; 2023

SAFIS

Introduction: The establishment of a successful pregnancy requires proper development of placental vasculature, which includes macrovasculature and microvasculature, and the coordinated regulation of vascular processes. In this context, the placenta can act as a sensor of fetal metabolic demands, inducing changes in its vasculature to ensure fetal growth and wellbeing. Cellular stress during pregnancy, including hyperosmolar stress, can impact the normal development of placental vasculature. Caveolin-l (Cav-l), a constitutive protein of caveolae, plays a pivotal role in cell signaling. Our hypothesis is that hyperosmolar stress disrupts placental angiogenesis, and Cav-l participates in this process. Objective: Our objective was to assess the effect of hyperosmolarity on placental migration and tubulogenesis, along with the expression of Cav-l under these conditions. Materials and Methods: This study received approval from the ethics committee of the Hospital Nacional Prof. Dr. A. Posadas. Placental microvascular endothelial cells (hPMEC) and the EA.hy926 cell line (ATCC@ CRL-2922TM ) were used. Cells were treated with a sucrose solution (100 mM) to induce hyperosmolarity. Cav-l expression was evaluated through RT-qPCR and western blot analysis. Cell migration was assessed via wound healing assays, and angiogenesis was evaluated through tube formation assays. Results: Hyperosmolar stress significantly reduced cell migration (p