OSMOTIC STRESS AND CAVEOLIN-1 EXPRESSION IN AMNIOTIC CELLS

BERTON, MAGALÍ; FERNANDEZ, NAZARENA; SIERRA, MATÍAS; COROMINAS ANA; DAMIANO ALICIA E; CASTRO-PARODI MAURICIO

Mar del Plata

Congreso; Reunion Anual de las Sociedades de Biociencias; 2022

SAIC-SAI-SAFIS

Osmotic stress and Caveolin-1 expression in amniotic cells Amniotic fluid is essential for normal fetal growth and development. Emerging evidence shows that the primary mechanism that regulates the intramembranous flow is an active unidirectional vesicular transport of water and solutes across the amnion cell It has been proposed that this transport process is mediated by transcytosis of caveolae-like vesicles. Oligohydramnios is a pregnancy pathology in which amniotic fluid volume decreases, increasing the osmolarity. This pathology is usually associated with preeclampsia in which we previously observed a decrease in caveolae in trophoblast cells.We hypothesize that hyperosmolarity alters the number of caveolae affecting the expression of caveolin-1 (Cav-1). Our objective was to evaluate the expression of caveolin 1 in amniotic and trophoblast cells exposed to hyperosmolarity. This study was approved by the ethics committee of the Hospital Nacional Dr. Prof. Alejandro Posadas. Human amnion and trophoblast explants were cultured in complete DMEM-F12 and sucrose hyperosmolar solutions were added to generate the hyperosmolar conditions. Cav-1 expression was analyzed by Western Blot analysis and its localization by immunohistochemistry.Results: In villus explants cultured in hyperosmolarity, we observed a 2.2-fold increase in caveolin 1 expression (p=0.0124, n=3), while no significant changes were observed in amniotic explants (p=0.08, n=3). The labeling of Cav-1 was found in the plasma membrane. Conclusions: Our results suggest that vesiculartransport in the amnion would not be mediated by caveolae-like vesicles in hyperosmolarity. More studies are necessary to evaluate the increase of Cav-1 in trophoblast cells in this osmotic condition.