INVESTIGADORES
CROCENZI Fernando Ariel
congresos y reuniones científicas
Título:
TAUROURSODESOXICOLATE (TUDC) PREVENTS THE CHOLESTATIC EFFECTS OF ESTRADIOL 17ß-D-GLUCURONIDE (E17G) VIA ACTIVATION OF Ca2+/CALMODULIN PROTEIN KINASE II (CaMKII).
Autor/es:
MEDEOT, ANABELA CAROLINA; BOAGLIO, ANDREA C.; RAZORI, MARÍA VALERIA; SALAS, GIMENA; SCHUCK, VIRGINIA SOLEDAD; CROCENZI, FERNANDO ARIEL; ROMA, MARCELO GABRIEL
Lugar:
Virtual
Reunión:
Congreso; Reunión de Sociedades de Biociencias 2020; 2020
Institución organizadora:
SAIC/SAFIS/SAI
Resumen:
E17G, the main etiologic agent of pregnancy-induced cholestasis, triggers bile flow drop by inducing endocytosis and intracellular retention of the canalicular transporters involved in bile secretion Mrp2 and Bsep, via activation of cPKC and PI3K, respectively. We showed that TUDC, the active metabolite of ursodeoxycholic acid (the most effective drug used to treat this condition), counteracts all these pathomechanisms. However, the signaling mediators in- volved in this protection remains unknown. Since TUDC increas- es cytosolic Ca2+ and, by doing so, promotes vesicular exocytosis under cholestatic conditions, we tested in rat hepatocyte couplets (RHC) whether Ca2+ and its downstream effector CaMKII mediate TUDC protective effects. E17G (200 μM, 20 min) decreased by 56±1 % and 60±2 % (p
