Beta-adrenergic inhibitory effect of alloimmune antibody on isolated oviductal tract of mice.

BORDA ES; GENARO AM; CREMASCHI GA; SALES ME; STERIN-BORDA L

EUROPEAN JOURNAL OF PHARMACOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 1984 vol. 100 p. 195 - 200

0014-2999

The effects of IgG purified from BALB/c anti CF1 sera on the spontaneous contractions of isolated oviductal tract from CF1 mice were explored. Cumulative dose-response curves were constructed for the effect of immune IgG on nice oviductal tracts from proestrus, estrus, metestrus and diestrus, comparing them with those obtained with norepinephrine. Both the adrenergic agonist and the immune IgG produced a sustained inhibition of spontaneous motility during the whole sex cycle. Normal IgG was virtually devoid of activity. The sensitivity of CF1 mouse oviducts to the inhibitory actions of immune IgG and norepinephrine varied depending on the hormonal stage, i.e. it was higher in natural diestrus than in metestrus; it became smaller in proestrus and was minimal during estrus. The mechanism triggered involved a beta-adrenergic reaction that could be blocked by 10(-7) M (-)-propranolol and 10(-6) M butoxamine and potentiated by chemical sympathectomy of the mice with 6-hydroxydopamine. It is concluded that: (a) alloimmune antibody reacts with isolated oviductal tract of mice inducing functional changes; (b) this action could be associated with an activation of postsynaptic beta-adrenergic sites of the plasma membrane and (c) the different effectiveness of the immune IgG observed during the sex cycle appears to depend on the affinity of beta-adrenoreceptors to react with it.