Altered ß-adrenoceptor function associated to PKC activation in hyperproliferative T lymphocytes

GA CREMASCHI; GENARO, ANA; C CAZAUX; C ANESINI; M WALD; L STERIN-BORDA

JOURNAL OF NEUROIMMUNOLOGY

Elsevier/North-Holland

Lugar: Netherlands; Año: 2000 vol. 110 p. 57 - 65

0165-5728

beta-Adrenoceptor (betaAR) expression and function as well as its modulation via intracellular transduction signals, were analyzed on the T cell lymphoma BW5147. Independently to the kinetic of proliferation and relative to the number of receptors displayed in normal T lymphocytes, BW5147 cells displayed a decreased number of betaAR, uncoupled to adenylate cyclase, but coupled to protein kinase C stimulation. This last effect was impaired by a beta-antagonist and by blockers of the enzymatic pathways involved in T lymphocyte proliferation, inducing a recovery of betaAR sites. Down-regulation of betaAR would implicate the loss of a negative neuroimmune control mechanism for lymphocyte proliferation. The coupling of the remaining sites to a positive signal for cellular activation, would contribute to establish an hyperproliferative state.