INVESTIGADORES
CORTON Eduardo
congresos y reuniones científicas
Título:
Towards the Development of Ferricyanide-mediated Electrochemical Rapid-BOD Biosensors
Autor/es:
BONETTO, M. C.; SACCO, N.; WEINSTOCK, A.; FRAIGI, L.; EDUARDO CORTON
Lugar:
Rosario
Reunión:
Congreso; V Congreso Argentino de Microbiología General (SAMIGE); 2008
Resumen:
<!-- /* Font Definitions */ @font-face {font-family:Calibri; mso-font-alt:"Century Gothic"; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; text-align:justify; line-height:150%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:Calibri; mso-bidi-font-family:"Times New Roman"; mso-fareast-language:EN-US;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> A ferricyanide-mediated rapid BOD approach has been reported to overcome the oxygen solubility in water limitation that contributes to long incubation time (5 days) in the standard BOD test (BOD5), internationally employed as a water quality parameter. BOD test is used to determine organic pollution in natural waters and the total degradable organic load in wastewaters. Here we present results obtained using free bacterial cells, in a batch system. Our results show that one of the bacterial strains tested reduces ferricyanide efficiently and is able to oxidize several carbon sources. These results are going to be used in the design of a microbial biosensor that, proficient for BOD rapid determination, will improve and facilitate active intervention in environmental monitoring. The development of rapid methods to be used in-situ, based in disposable electrochemical strips, is our ultimate goal.