INVESTIGADORES
CORTON Eduardo
artículos
Título:
A Simple Laser-Based Device for Simultaneous Microbial Culture and Absorbance Measurement
Autor/es:
ABREVAYA X. C.; CORTÓN, EDUARDO; ARESO OMAR; MAUAS P. J. D.
Revista:
INSTRUMENTS AND EXPERIMENTAL TECHNIQUES
Editorial:
MAIK NAUKA/INTERPERIODICA/SPRINGER
Referencias:
Lugar: Kiev; Año: 2013 vol. 56 p. 472 - 476
ISSN:
0020-4412
Resumen:
In this work we present a new device specifically designed to study microbial growth. It can measure simultaneously and consistently up to16 samples (which are mixed by a circular wheel), that could be installedinside an incubator or directly in contact with natural environmental conditions. The absorbance data is acquired, processed, and exported to a PC for the generation of microbiological growth curves, and the results are available remotely via the Internet. The linear working range of the AMC-d was determined, and its reliability was tested by comparison with measurements performed in a commercial spectrophotometer. The applicability of the AMC-d was studied using two different microbialcultures which have very different generation times: Escherichia coli,a bacterium commonly used in research, and the extremophilic organismHaloferax volcanii, an halophilic archaeon.
