Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay

BONETTO, M. C.; SACCO, N.; ASTRID HILDING OHLSSON; EDUARDO CORTON

TALANTA

ELSEVIER SCIENCE BV

Lugar: Londres; Año: 2011 vol. 85 p. 455 - 462

0039-9140

Methods for short-term BOD analysis (BODst) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard BOD test analysis (BOD5) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. Here we present a bioassay where a Klebsiella pneumoniae environmental strain successfully reduces ferricyanide without de-aeration of the samples with linear BOD5 ranges between 30 and 500 mg L-1 or 30 and 200 mg L-1, using glucose-glutamic acid solution (GGA) or OECD standards respectively. We further propose a new assay termination solution that allows higher reproducibility and standardization of the cell-based assay, employing formaldehyde (22.7 g L-1) or other compounds in order to stop ferricyanide reduction without affecting the amperometric detection and therefore replace the centrifugation step normally used to stop microbial-driven reactions in ferricyanide-mediated bioassays. These improvements led to an accurate determination of real municipal wastewater samples.