INVESTIGADORES
CORONADO Eduardo A.
artículos
Título:
Plasmonic sensing through bioconjugation of Ag nanoparticles: Towards the development of immunoassays for ultralow quantification of antigens in colloidal dispersions
Autor/es:
PABLO A. MERCADAL; JUAN C. FRAIRE; RUBEN MOTRICH; EDUARDO A. CORONADO
Revista:
Advaneced Materilas Letters
Editorial:
VBRI Press
Referencias:
Año: 2018 vol. 9 p. 456 - 461
ISSN:
0976-3961
Resumen:
he combination of the optical properties of silver nanoparticles with some of the tool-kits of the widespread used ELISAmethod for antigen quantification immunoassays, give rise to an enzyme free, low cost, fast and more sensitive opticalmethod denoted as Intensity Depletion Immuno-linked Assay (IDILA), that can be performed directly in colloidal dispersionswithout any immobilization of the capture antibody, antigen, secondary and primary antibodies on a substrate. Thecapabilities of the method for quantifying antigens at ultralow concentrations in colloidal dispersions as well as itsperformance are demonstrated for specific antigens of importance in medicine and in food science.
