INVESTIGADORES
CONFALONIERI Viviana Andrea
artículos
Título:
Complex mutational patterns and size homoplasy at maize microsatellite loci.
Autor/es:
LIA, V.V.; BRACCO, M.; GOTTLIEB, A.M.; POGGIO, L.; CONFALONIERI, V.A.
Revista:
Theor. Appl. Genet.
Editorial:
Springer
Referencias:
Año: 2007 vol. 115 p. 981 - 991
Resumen:
Microsatellite markers have become one of the
most popular tools for germplasm characterization, population
genetics and evolutionary studies. To investigate the
mutational mechanisms of maize microsatellites, nucleotide
sequence information was obtained for ten loci. In addition,
Single-Strand Conformation Polymorphism (SSCP) analysis
was conducted to assess the occurrence of size homoplasy.
Sequence analysis of 54 alleles revealed a complex
pattern of mutation at 8/10 loci, with only 2 loci showing
allele variation strictly consistent with stepwise mutations.
The overall allelic diversity resulted from changes in the
number of repeat units, base substitutions, and indels within
repetitive and non-repetitive segments. Thirty-one electromorphs
sampled from six maize landraces were considered
for SSCP analysis. The number of conformers per electromorph
ranged from 1 to 7, with 74.2% of the electromorphs
showing more than one conformer. Size homoplasy was
apparent within landraces and populations. Variation in the
amount of size homoplasy was observed within and
between loci, although no diVerences were detected among
populations. The results of the present study provide useful
information on the interpretation of genetic data derived
from microsatellite markers. Further eVorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
populations. The results of the present study provide useful
information on the interpretation of genetic data derived
from microsatellite markers. Further eVorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
Verences were detected among
populations. The results of the present study provide useful
information on the interpretation of genetic data derived
from microsatellite markers. Further eVorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
Vorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
ranged from 1 to 7, with 74.2% of the electromorphs
showing more than one conformer. Size homoplasy was
apparent within landraces and populations. Variation in the
amount of size homoplasy was observed within and
between loci, although no diVerences were detected among
populations. The results of the present study provide useful
information on the interpretation of genetic data derived
from microsatellite markers. Further eVorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
populations. The results of the present study provide useful
information on the interpretation of genetic data derived
from microsatellite markers. Further eVorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
Verences were detected among
populations. The results of the present study provide useful
information on the interpretation of genetic data derived
from microsatellite markers. Further eVorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
Vorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
per electromorph
ranged from 1 to 7, with 74.2% of the electromorphs
showing more than one conformer. Size homoplasy was
apparent within landraces and populations. Variation in the
amount of size homoplasy was observed within and
between loci, although no diVerences were detected among
populations. The results of the present study provide useful
information on the interpretation of genetic data derived
from microsatellite markers. Further eVorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
populations. The results of the present study provide useful
information on the interpretation of genetic data derived
from microsatellite markers. Further eVorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
Verences were detected among
populations. The results of the present study provide useful
information on the interpretation of genetic data derived
from microsatellite markers. Further eVorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
Vorts are still needed
to determine the impact of these Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
of population parameters and on the inference of phylogenetic
relationships in maize investigations.
Wndings on the estimation
of population parameters and on the inference of phylogenetic
relationships in maize investigations.