INVESTIGADORES
COLOMBO Maria Isabel
artículos
Título:
A phorbol ester-binding protein is required downstream of Rab5 in endosome fusion
Autor/es:
ABALLAY, ALEJANDRO; BARBIERI, MA; ARENAS, N; PD STAHL; LS MAYORGA; MARIA ISABEL COLOMBO
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 1998 vol. 441 p. 373 - 378
ISSN:
0014-5793
Resumen:
Previous observations indicate that a zinc and phorbol ester binding factor is necessary for endosome fusion. To further characterize the role of this factor in the process, we used an in vitro endosome fusion assay supplemented with recombinant Rab5 proteins. Both zinc depletion and addition of calphostin C, an inhibitor of protein kinase C, inhibited endosome fusion in the presence of active Rab5. Addition of the phorbol ester PMA (phorbol 12-myristate 13-acetate) reversed the inhibition of endosome fusion caused by a Rab5 negative mutant. Moreover, PMA stimulated fusion in the presence of Rab5 immunodepleted cytosol. These results suggest that the phorbol ester binding protein is acting downstream of Rab5 in endosome fusion.
