Cathepsin L3 promotes IFN- response by CD8 T cells during Fasciola hepatica infection

CELIAS D; SILVANE L; CORVO I; TORT J; MOTRAN,CC; . CERVI L.

Mar del Plata

Congreso; SAIC-SAI-SAFE 2016. LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2016

SAIC-SAI-SAFE

(716) CATHEPSIN L3 PROMOTES IFN-γ RESPONSE BYCD8 T CELLS DURING FASCIOLA HEPATICA INFECTIONDaiana Pamela Celias1, Leonardo Micael Silvane1, IleanaCorvo2, José Tort3, Cristina Motrán1, Laura Cervi1.1Departamento de Bioquímica Clínica Facultad de CienciasQuímicas. Universidad Nacional de Córdoba. CIBICICONICET.Córdoba, Argentina. 2Laboratorio de I+D deMoléculas Bioactivas, Centro Universitario de Paysandú,Universidad de la República, Uruguay. 3Facultad de Medicina.Universidad de la República, Uruguay.During its migration through host tissue, the helminth parasiteF. hepatica, secretes a number of distinct cathepsin L cysteinepeptidases. Among them, cathepsin L3 (CL3) is highly expressedin the juvenile larvae, which has the ability to degrade collagen.However, the effect of CL3 on the modulation of immune responseis still unknown. The aim of this work was to study the ability ofCL3 to induce an immune response during F. hepatica infectionin mice. A role of dendritic cells (DC) in this modulation was alsoinvestigated. C57BL/6 mice (B6) were orally infected with 12 F.hepatica metacercariae per animal. After 4 and 17 days postinfection peritoneal cells (PECs) and splenocytes were removed,respectively. These cells were cultured in presence or absence ofCL3 or total parasite lysates (TE) for 48h. Cytokines productionwas detected by ELISA or FACS analysis. Moreover, DC weredifferentiated from B6 bone marrow with GM-CSF and culturedfor 18 h with CL3 (produced in Hansenula polymorpha) or LPS,and injected in B6. After 7 days lymph nodes were obtained andstimulated with CL3. Both PECs and splenocytes from infectedanimals restimulated with CL3, secrete significantly higher levels ofIFN-γ and IL-17 than those observed in uninfected mice (p