Role of programmed death ligand 2 in macrophage activation during Fasciola hepatica infection.

STEMPIN, C; FALCON CR; AOKI P; MOTRAN CC; CERBAN F; CERVI L,

Mar de Plata

Congreso; LXII Reunion anual Sociedad Argentina de Inmunología; 2014

Sociedad Argentina de Inmunología

(823) ROLE OF PROGRAMMED DEATH LIGAND 2 IN MACROPHAGE ACTIVATION DURING FASCIOLA HEPATICA INFECTION Stempin, Cinthia C; Falcon, Cristian R.; Aoki, María Del Pilar; Motrán, Claudia C.; Cerban, Fabio M.; Cervi, Laura Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI)-CONICET-UNC Helminth infections induce an increase in co-inhibitory molecule programmed death ligand-2 (PD-L2) which is defied as a marker for alternatively activated macrophages and is involved in the inhibition of T cell proliferation. However, it is not known how PD-L2 modulates macrophage activation, which promotes firosis in helminth infections, whereas classical activation produces deleterious toxic metabolites. Previously we have observed that F. hepatica infection increases PD-L2 expression in F4/80+ cells. The aim of this study was to investigate the role of PD-L2 in the outcome of F. hepatica infection as well as in macrophage activation during experimental infection with the parasite. BALB/c WT and PD-L2 KO were orally infected with 8 F. hepatica metacercariae. Survival rate was evaluated and histological changes in liver tissues were determined by hematoxylin and eosin staining. PD-L2 KO mice showed increased mortality during F. hepatica infection (p