Superinfection with two different strains of HIV-1: reactivation, pseudotype production and superinfecting strain recovery

.PABLO N. FERNÁNDEZ LARROSA, ,; ANA CEBALLOS; SILVIA MARQUINA; MARTINEZ PERALTA; 9.PABLO N. FERNÁNDEZ LARROSA, CEBALLOS A, SILVIA MARQUINA, LILIANA MARTÍNEZ PERALTA, ROBERTO D. RABINOVICH.

Arch Virol

SPRINGER

Año: 2005 vol. 15 p. 651 - 662

Arch Virol. 2006 Apr;151(4):651-62. Epub 2005 Dec 15. Viral reactivation and pseudotype production in an in vitro superinfectionsystem with two different strains of HIV-1.Fernandez Larrosa PN, Ceballos A, Andreani G, Marquina S, Martinez Peralta L,Rabinovich RD.National Reference Center for AIDS, Department of Microbiology, University ofBuenos Aires, School of Medicine, Buenos Aires, Argentina. plarrosa@fmed.uba.arViral production and variability of HIV-1 is normally high in vivo causing thenecessary conditions for cellular superinfection. In order to evaluate thesuperinfection dynamics in vitro, H9HTLVIIIB cell line was superinfected withHIVMN. Superinfected cells showed nearly 50% cell mortality at day 1post-superinfection (ps), which increased significantly up to day 4 ps.Superinfecting genome was detectable until day 10 ps. The superinfecting strainwas found in the supernatant only on day 1 ps, but was recovered up to day 4 psby coculture with non-infected cells. The existing strain (HIVHXB2) wasrecovered throughout the studied period. Pseudotype formation by the HIVHXB2genome and envelope proteins of the superinfecting strain (HIVMN) was observedfrom day 1 to 6 ps. Viral production was increased by 1.7 LOG in superinfectedcells from day 1 ps. Both viral production increase and pseudotype formationcould be relevant for HIV pathogenesis in vivo.