Detection of HIV-1 dual infections in highly exposed 1 treated patients

ANDREANI G*, PETRONI A, PUGLIESE D , BOUZASMB, FERNANDEZ GIULIANO S, WEISSENBACHER, MC, LOSSO M , BENETUCCI J, CARR JK MARTÍNEZ PERALTA L,* THESE AUTHORS CONTRIBUTED EQUALLY TO THIS STUDY.; ESPADA COTI*; ANA CEBALLOS*; AMBROSIONE JUAN; PETRONI; BENETUCCI J; CARR J; LILIANA MARTINEZ PERALTA

VIROLOGY JOURNAL

BIOMED CENTRAL LTD

Año: 2011 p. 392 - 398

1743-422X

Background: Genetic characterization of HIV-1 in Argentina has shown that BFGenetic characterization of HIV-1 in Argentina has shown that BF 23 recombinants predominate among heterosexuals and injecting drug users, while in men whorecombinants predominate among heterosexuals and injecting drug users, while in men who 24 have sex with men the most prevalent form is subtype B.have sex with men the most prevalent form is subtype B. 25 Objectives: The aim of this work was to investigate the presence of HIV dual infections inObjectives: The aim of this work was to investigate the presence of HIV dual infections in 26 HIV-infected individuals with high probability of reinfection.HIV-infected individuals with high probability of reinfection. 27 Study design: Blood samples were collected from 23 HIV positive patients with the risk ofStudy design: Blood samples were collected from 23 HIV positive patients with the risk of 28 reinfection from Buenos Aires. A fragment of the HIV gene pol was amplified andreinfection from Buenos Aires. A fragment of the HIV gene pol was amplified and 29 phylogenetic analyses were performed. Antiretroviral drug resistance patterns of all thephylogenetic analyses were performed. Antiretroviral drug resistance patterns of all the 30 sequences were analyzed.sequences were analyzed. 31 Results: Five dual infections were detected with four patients coinfected with subtype BResults: Five dual infections were detected with four patients coinfected with subtype B 32 and BF recombinants and one patient was coinfected with two BF recombinants presentingand BF recombinants and one patient was coinfected with two BF recombinants presenting 33 different recombination patterns. Prolonged infection with a stable clinical condition wasdifferent recombination patterns. Prolonged infection with a stable clinical condition was 34 observed in the five individuals. Resistance mutation patterns were different between theobserved in the five individuals. Resistance mutation patterns were different between the 35 predominant and the minority strains.predominant and the minority strains. 36 Conclusions: Our results show that HIV dual infection can occur with closely related subtypes,Conclusions: Our results show that HIV dual infection can occur with closely related subtypes, 37 and even with different variants of the same recombinant form in certain populations. Clinicaland even with different variants of the same recombinant form in certain populations. Clinical 38 observations showed neither aggressive disease progression nor impact on the resistance patterns inobservations showed neither aggressive disease progression nor impact on the resistance patterns in 39 the dually-infected patients.the dually-infected patients. 40