INVESTIGADORES
CAVALITTO Sebastian Fernando
artículos
Título:
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876
Autor/es:
CAVELLO, IVANA; HOURS, ROQUE ALBERTO; CAVALITTO, SEBASTIÁN FERNANDO
Revista:
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Editorial:
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Referencias:
Lugar: Seul; Año: 2013 vol. 23
ISSN:
1017-7825
Resumen:
Enzymatic
decomposition of gelatin layers on used X-ray films and repeated
utilization of enzyme for potential application in silver recovery were
investigated using keratinolytic serine proteases from Purpureocillium lilacinum LPS
# 876. At pH 9.0, the enzymatic reaction was enhanced by the increase
of enzyme concentration or by the increase of the temperature up to 60
ºC. Under the conditions of 6.9 U/ml, 60 ºC and pH 9.0, hydrolysis of
gelatin layers and the resulting release of silver particles were
achieved within 6 min. The protective effect of polyols against thermal
denaturation was investigated. The presence of glycerol and propylene
glycol increases enzyme stability. When the reusability of the enzyme
for gelatin hydrolysis was tested it could be seen that it could be
effectively reused for more cycles when glycerol was added compared to
the enzyme without protective agents. The results of these repeated
treatments suggested that a continuous process of recycling silver from
used X-ray is feasible. Keeping in mind that recycle is -at the
present time- needed and imperative, it can be remark that, in this
research, three wastes were used: hair waste in order to produce serine
proteases; glycerol in order to enhance enzyme thermal stability and
used X-ray films in order to recover silver and PET films.