INVESTIGADORES
CATALDI Angel Adrian
congresos y reuniones científicas
Título:
Intranasal vaccination of pregnant dams with Intimin and EspB protects neonatal mice from Escherichia coli (EHEC) O157:H7 infection.
Autor/es:
RABINOVITZ BC, ; LARZÁBAL M, ; VILTE DA, ; CATALDI A, ; MERCADO EC.
Reunión:
Simposio; VTEC 2012; 2012
Resumen:
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Protection against enterohemorragic E. coli conferred to the
offspring though vaccination of mothers in a murine model
Rabinovitz, Cataldi, Palermo, Mercado, Vilte
Intranasal vaccination of
pregnant dams with Intimin and EspB protects neonatal mice from Escherichia coli (EHEC) O157:H7
infection
Rabinovitz BC1,
Larzábal M2, Vilte DA1, Cataldi A2, Mercado EC1
1Instituto de
Patobiología and 2Instituto de Biotecnología, CICVyA, Instituto
Nacional de Tecnología Agropecuaria, Hurlingham, Buenos Aires, Argentina
Enterohemorrhagic Escherichia
coli (EHEC) O157:H7 are responsible
for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic
complication which particularly affects children. A number of different mouse
models for EHEC O157:H7 infection have been developed. In this study we
evaluate whether passive immunization protects from EHEC O157:H7 colonization
and renal damage, using a
weaned BALB/c mice model of infection. Recombinants
proteins EspB and the carboxyl-terminal fragment of 280 amino acids of
γ-intimin (γ-Int C280) were used as immunogens in
combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant. Two
groups of six pregnant mice were immunized three times by the intranasal route
with 20 μg of γ-Int C280
or EspB, respectively, formulated in 10 μl of PBS and mixed with 5
μg/dose of adjuvant. A control group received PBS mixed with adjuvant. Neonatal mice were allowed to
suckle vaccinated or sham-vaccinated dams until weaning (17- 21 days of age,
8-11g of body weight) when they were challenged by the oral route with a
suspension of 1x107 CFU of an E. coli O157:H7 Stx2+ strain. Rectal swabs were taken at 48 and 72 h after
infection to determine the excretion of EHEC O157:H7 organisms by
bacteriological counts. Neonatal mice were necropsied five days after inoculation and intestinal and kidney samples were collected for
histopathological analysis. Pathogenic effects of the challenge strain were evaluated by mortality
rate and plasmatic urea levels. γ-IntC280 and EspB IgG titers in dams[AC1] and weaned mice were determined by an enzyme-linked immunosorbent
assay (ELISA). Statistical differences were determined using one-way analysis of
variance. All vaccinated dams exhibited elevated serum IgG response against
both γ-Int C280 and EspB. Passive immunization resulted in a
significant increase in serum IgG titers against γ-Int C280 and
a slight increase in EspB- specific antibodies in the neonatal mice. All the weaned mice remained clinically healthy
following inoculation with EHEC O157:H7 and renal or intestinal lesions were
not observed when the animals were necropsied on day five after bacterial
challenge. However, neonates nursing vaccinated dams showed a significant
reduction in EHEC O157:H7 colonization at 48 h post challenge. In addition, the
level of plasma urea concentration , a clinical parameter of systemic effect of
EHEC O157:H7 infection, was significantly higher in the control group. In
conclusion, passive immunization with antibodies against γ-Int C280 or
EspB could reduce EHEC O157:H7 colonization and renal effects of EHEC
toxicity.
